Thiofluor

N,N-Dimethyl-2-mercaptoethylamine hydrochloride
Chromatographic Grade™

For Automated Post-column Derivatization of Primary Amines

Primary amines form highly fluorescent adducts when reacted with o-Phthalaldehyde (OPA) and a mercaptan under basic conditions.

The products of this reaction, 1-alkyl-2-thioalkyl-subsitituted isoindoles, exhibit optimal excitation at 330 nm and maximal emission at 465 nm.

Pickering's Thiofluor™, a solid, nearly odorless nucleophile, is a superior substitute for 2-Mercaptoethanol in the preparation of OPA reagents.  It forms a more stable reagent and a longer-lasting fluorophore with OPA than does 2-Mercaptoethanol, yet it has the same fluorescence properties.

Unlike the volatile 2-Mercaptoethanol, Thiofluor™ will not migrate through the gas manifold and regulator of the OPA reagent pressurization system.

Two grams of Thiofluor™ is equivalent to 1 mL of 2-Mercaptoethanol.