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TRIONE Ninhydrin Reagents
TRIONE® NINHYDRIN REAGENT
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Chromatographic Grade™
A prepared Reagent for Automated Post-column Derivatization of Primary and Secondary Amines
TRIONE® Ninhydrin reagent is specially formulated for amino acid analysis. It contains Ninhydrin, Hydrindantin (reduced Ninhydrin), a Lithium Acetate buffer, and Sulfolane, a water-miscible organic solvent. The solvent is necessary to maintain the solubility of both the Hydrindantin and the primary amine product, Ruhemann's Purple. The buffer is required because the reaction is pH dependent. The active ingredients—Ninhydrin and Hydrindantin are required for proper development of secondary and primary amines, respectively.
TRIONE® is so stable that is does not require refrigeration, either in shipment, storage, or in the reservoir. Quantitation is consistent from the first to the last mL, so there is no waste. The high signal-to-noise ratio of TRIONE®, when compared to DMSO-containing reagents, permits detection sensitivity to be increased with minimum increase in background noise—a feature particularly appreciated at sample concentrations of <50 pico moles. |
Two Preparations are available to suit your usage and storage requirements:
T100/T100C
- Pour into your reservoir and use; the ultimate in convenience with a minimum of handling
- 3-month* shelf life
T200
- Combine two solutions, swirl, and use
- 12-month* shelf life before mixing preparation; one month in the reservoir
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TRIONE Ninhydrin Reagent (3-month* shelf life), each (950 mL/bottle)
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TRIONE Ninhydrin Reagent (3-month* shelf life), case of 4(950 mL/bottle)
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TRIONE Two-part Ninhydrin Reagent (12-month shelf life* before mixing), to prepare, case of 4 (900 mL/bottle) * From date of manufacture
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